Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Brain and behavior

Article Title: The behavioral and physiological effects of high-fat diet and alcohol consumption: Sex differences in C57BL6/J mice.

doi: 10.1002/brb3.708

Figure Lengend Snippet: FIGURE 6 Whole-brain BDNF. When normalized to their respective sex controls, males overall exhibited small but significant decreases to BDNF compared to females. Black bars indicate water, and gray bars indicate 10% EtOH. The dashed line indicates 100% RC H2O expression. ‡ significant sex difference at p < .05

Article Snippet: A low target concentration (working dilution 1:2) of sample and sample diluent buffer was created and then used in a BDNF ELISA (Mouse BDNF PicoKine ELISA Kit, Boster Biological Technology Co., Pleasanton, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Quantitative Analysis of Focused A-To-I RNA Editing Sites by Ultra-High-Throughput Sequencing in Psychiatric Disorders

doi: 10.1371/journal.pone.0043227

Figure Lengend Snippet: A . Shows the distribution of A-to-I RNA editing frequency of 36 sites from category I, including 11 of the ‘new’ sites identified by Li et al (ref 24) and 25 previously ‘known’ sites, in the cortex and cerebellum from two sets of normal human brain samples. The RNA editing frequency is presented as a percentage of the total population of transcripts. A two-fold increase of RNA editing frequency at 6 sites (CCNI (R/G), CCNI (K/R), 5HT2C (siteB), 5HT2C (siteC), 5HT2C (siteE) and TRO) in cortex and one site (FLNB) in cerebellum are shown in the inset. B . Shows the distribution of A-to-I RNA editing frequency of 36 sites from category I, in the anterior temporal cortex (ATC) and the right frontal cortex (RFC) from the third set of normal human samples (n = 5). The RNA editing frequency is presented as a percentage of the total population of transcripts. No significant difference was found between these two regions of cortex.

Article Snippet: Human cortex and cerebellum total RNA from pooled normal human control samples (n = 10) were obtained from Clontech.

Techniques: